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with SONOPULS HD 2000/2000.2/3000/4000 series ultrasonic homogenisers.
Ultrasonic homogenizers
SONOPULS Ultrasonic homogenisers are mainly used for homogenising, desagglomerising, emulsifying, suspending, acceleration of chemical reactions as well
as for disruption of cells, bacteria, fungi or spores. Significantly reduced processing times and quickly available results make the ultrasonic homogenisers indispensable for modern processes and in analysis. Using ultrasonic homogenisers certain substances can be selectively destroyed, tedious preparation processes can be shortened and results of many reactions are improved. When sonicating samples not only μm particles
can be achieved but also particles in nm range! With many years of experience in the field of ultrasonic homogenisers we assist you in your specific application.
SONOPULS homogenizers with amplitude regulation 10 to 100 % and actual value display, pulse rate, AMPLICHRON® mode (guarantees a constant amplitude in independence of changing conditions), integrated timer, remote control. All units have a CE mark and are medical products according to the guideline for in vitro diagnostics 98/79/EG.
Fields of application:
- Disruption of cells, bacteria, virus, tissue
- Production of finest emulsions
- Homogenization of substances
- Degassing of fluids
- Sample preparation for particle size analysis
- Acceleration of chemical reactions
- Sample preparation for waste-water analysis
For more information visit our page about the ultrasonic homogenizers in laboratory use, check our laboratory catalogue and our application guide.
SONOPULS mini20
SPECIAL
For volumes from 0.1 to 25 ml
Ready-to-use set:
for volumes from 0.5 to 25 ml
ultrasonic nominal output max. 20 W
• Ultrasonic generator mini20
• Ultrasonic converter mini20
• Microtip MS 2.5, dia. 2.5 mm
HD 4100
ADVANCED
Sonication for liquid volumes from 0.5 ml to 3.000 ml in stationary run and up to 30 l/h in flow-through operation.
Sound proof box LS 40
Accessories and additional equipment
Accessoires for the multiple applications of the SONOPULS units.
History
Applications
Typical areas of application
• Disruption of cells without distroying the cell content
• Disruption of tissue, also mixed tissue
• Emulsifying of hardly mixable liquids, e. g. oil and
water, particle size in nm range
• Deagglomeration of nanoparticles in material
research (nanostructurised material) in medicine,
biotechnology, automobile industry
• Acceleration of chemical reactions
• Dispersing
Analysis
• Preparing samples for grain size determination or
environmental analysis
• Homogenising of cheese samples for determination
of nitrates
Biochemistry – Biology – Medicine
• Sonication of small high-quality samples for analysis
like EIA or RIA
• Due to high amplitudes, disruption of either highresistant
bacteria, cells are tissues is possible.
Indirect processing of samples in cup booster BR 30
or in cup horn BB 6 are recommended to avoid
crosscontamination
• Detection of prions by cyclic amplification of protein
misfolding
Chemistry – Sonochemistry
• Acceleration of chemical reactions or destroying of
highly-molecular compounds
Pharmacy – Cosmetics
• Production of larger volumes of long lasting emulsions,
e g. lotions and production of antigens,
vaccines or liposomes
General information (extract)
5119 General information
on ultrasonic homogenisers
5169 Power determination
5159 Life span of probes
5972 Application guide
Molecular Biology – Microbiology – Pharmacy
– Medicine
B-101 Protein extraction by indirect sonication
B-102 Disruption of yeasts cells
B-103 Procurement of stroma-free haemolysate /
paternity test
B-106 Tissue disruption, especially „difficult“ tissues
– overview
B-108T Escherichia coli
B-109 Disruption of pseudomonas thailandensis
B-111 Protein isolation for Westernblot
B-207 Cell disruption of micro alges and
cyano bacteria
B-209 Producing of lysates of eucaryontic cells
Materials
C-104 Dispersing of carbon nanotubes (CNT) in
processing oil
C-203 Sample preparation of ceramic suspensions
for measuring the particle size
C-209 Phase transfer of ferric oxides nanoparticles
Environment
C-106 Desagglomeration of water and
sediment samples
C-110 Preparation of sewage samples
C-201 Extraction of magnesium out of soils
C-210 Sample preparation of sewage water for
determining of TOC according to DIN EN 1484
Typical Applications
Producing an oil / water emulsion
Small production of pharmaceutical formulations,
e.g. very fine emulsions like lotions
no agglomerates, no sedimentation
Homogenising of brain
Volume: 50 ml
Homogenising of Pangasius fish
Homogenising of cheese for subsequent
determining of nitrate
Essential aspects for choosing the appropirate
SONOPULS ultrasonic homogeniser
What is the difference between ultrasonic
homogenisers and ultrasonic baths?
The power [W] of ultrasonic baths is fixed. The power density [W/l] is relatively low.
Ultrasonic homogenisers have an adjustable power [W] and produce very high power densities [W/l]. Probes with a defined radiating surface guarantee reproducible results.
What is more important when choosing the appropriate
device – power rating or amplitude?
Power output [W] is not the sole criterion for selecting the ultrasonic homogeniser. This value only indicates the power of the ultrasonic generator but not the energy delivered into the sample. The amplitude at the radiating surface of the probe is the determining factor while considering the sample volume. SONOPULS homogenisers provide higher amplitudes than comparable devices in the market due to an optimal matching of all components.
Which information are necessary for an offer?
Application field
e. g.: homogenising, dispersing, extraction, cell disruption
Target of sonication
e. g.: isolation of cell content
Volume
batch operation of flow-through operation (quantity per
time unit)
Viscosity
in [mPas]
Solids content
suspensions in [%]
Temperature range
e. g.: temperature sensitive, cooling necessary
Components
e. g.: alcoholic or acidic